Mohammad Fathi; Reza Gharakhanlou; Masoud Soleimani; Hamid Rajabi
Volume 21, Issue 4 , 2019, Pages 1-8
Abstract
Background: The response of microRNA (miR-1), upstream regulators (Myogenic Differentiation 1 (MyoD1) and Myocyte Enhancer Factor 2C (Mef2C)), and a downstream target (Histone Deacetylase 4 (Hdac4)) to an acute bout of Resistance Exercise (RE) in Extensor Digitorum Longus (EDL) and soleus muscles has ...
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Background: The response of microRNA (miR-1), upstream regulators (Myogenic Differentiation 1 (MyoD1) and Myocyte Enhancer Factor 2C (Mef2C)), and a downstream target (Histone Deacetylase 4 (Hdac4)) to an acute bout of Resistance Exercise (RE) in Extensor Digitorum Longus (EDL) and soleus muscles has remained elusive.Objectives: In this experimental study, we investigated the effect of an acute bout of RE on the expression of miR-1, MyoD1, Mef2C, and Hdac4 genes in the slow and fast-twitch muscle of rats.Methods: The current study was conducted at Tarbiat Modares University, Tehran, Iran, in 2017. Fifteen male Wistar rats were ran- domly divided into two groups, control (n = 5) and RE (n = 10). The RE protocol consisted of four sets of five repetitions of climbing a ladder with weights attached to the tails of rats. The soleus and EDL muscles of rats were collected at 3 h (n = 5) and 6 h (n = 5) post-RE. The real-time polymerase chain reaction was used to measure the levels of miR-1, MyoD1, Mef2C, and Hdac4 mRNA expression.Results: The miR-1 expression in EDL muscle was significantly lower at 3 h and 6 h post-exercise in the RE group than in the control group (P < 0.01). The miR-1 expression in soleus muscle was significantly lower at 3 h post-exercise in the RE group than in the control group (P < 0.007) but it was significantly higher at 6 h post-exercise (P < 0.036). The expression of MyoD1, Mef2C, and Hdac4 genes in the EDL muscle was higher in the RE group than in the control group (P < 0.01). The expression of these genes in response to RE had more fluctuations in soleus muscle.Conclusions: It can be concluded that miR-1 expression in extensor digitorum longus and soleus muscles of rats responds to resis- tance exercise in different manners and this coincides with a change in upstream regulators and downstream target.
Mohammad Fathi; Nilofar Massoudi; Amirhesam Alirezaei; Mahmood Bakhtiyari
Volume 20, Issue 10 , 2018, Pages 1-8
Abstract
Background: Metabolic acidosis following kidney transplantation can lead to several undesirable effects such as disturbing the protein balance resulting in a negative nitrogen equilibrium, increased protein degradation, and essential amino acid oxidation. Objectives: The current study aimed tocompare ...
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Background: Metabolic acidosis following kidney transplantation can lead to several undesirable effects such as disturbing the protein balance resulting in a negative nitrogen equilibrium, increased protein degradation, and essential amino acid oxidation. Objectives: The current study aimed tocompare the effect of normal saline as a common crystalloid in kidney transplant surgery to reduce the risk of hyperkalemia and sodium bicarbonate on the metabolic acidosis rate after renal transplantation. Methods: A total of 40 patients with end-stage renal disease (ESRD) and candidates receiving renal transplant from a living donor referring to the kidney transplantation department of a University affiliated hospital, Tehran, Iran, that meeting the inclusion cri- teria of this study, were randomly assigned to the intervention group, who were treated with sodium bicarbonate, and the control group, who were treated with normal saline. The primary outcomes measured in this study were acidosis biomarkers including BE, HCO3, PaCO2, and PH and the secondary outcomes included some inflammatory biomarkers and some renal function biomarkers.Results: The mean ± standard deviation of age in the intervention and control groups was 44.4 ± 12.4 and 40.0 ± 13.0, respectively (P = 0.01). A total of 29 of the participants were male (14 in the intervention group and 15 in the control group), and 11 of them (six in the intervention group and five in the control group) were female. The mean ± standard deviation of change in the concentration of PH, BE, HCO3, and PaCO2 in the intervention and control groups was 0.01 ± 0.1 and -0.08 ± 0.1 (P < 0.001); 0.61 ± 4.2 and -3.8 ± 2.6 (P < 0.001); 0.36 ± 2.9 and -2.67 ± 2.1 (P: 0.001); and 0.8 ± 5.4 and 1.4 ± 5.3 (P: 0.799), respectively. Conclusions: The present study revealed that the patients who received sodium bicarbonate had a better status than the normal saline recipients in terms of the maintenance of acid-base homeostasis, inflammatory indexes, and renal function.
Majid Aliasgari; Amir Hesam Alirezaei; Nilofar Massoudi; Farid Dadkhah; Syd Ahmad Tara; Mohammad Fathi
Volume 19, Issue 3 , March 2017, , Pages 1-4
Abstract
Heart failure is common in patients with chronic renal disease, either as a complication of renal failure or from shared risk factors, or is the major cause of death in patients on dialysis. At present, end stage renal disease (ESRD) patients who have systolic heart failure are considered high-risk for ...
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Heart failure is common in patients with chronic renal disease, either as a complication of renal failure or from shared risk factors, or is the major cause of death in patients on dialysis. At present, end stage renal disease (ESRD) patients who have systolic heart failure are considered high-risk for surgery; and nephrologists and cardiologists are reluctant to refer these patients for kidney transplant evaluation. It is unclear whether such patients should be accepted and waitlisted for transplantation. seven cases with end stage renal disease (ESRD) and severe heart failure with ejection fraction (EF) of less than 20% and newyork class 3 - 4, despite being on optimal treatment, who underwent renal transplant from nonrelative living donor at Shahid Moddaress hospital in Tehran, Iran during the July 2013 to December 2015, were retrospectively collected and analysed. The mean ± SD of patient’s age was 32.7 ± 16 years, and about 72% of them were female. The left ventricle ejection fraction increased by 35% on an average after the renal transplantation. Renal transplantation significantly improved the LV systolic function and ejection fraction status and subsequently decreased the need for medical treatment and heart transplantation.
